Transfer the sections onto glass slides suitable for immunohistochemistry. Allow the slides to dry overnight and store slides at room temperature until ready for use.

My Antibody Suggestions These are the IHC approved antibodies that I have optimized for staining in the Oliver Lab. Some of them are ideal and others may need further optimization. I …

Sample preparation for IHC may include fixation, dehydration, embedding, and sectioning, but one or two experimental variables usually determine the exact workflow.

This step by step protocol provides everything you need to carry out successful immunohistochemistry.

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Critical steps in the recommended protocol – how critical are they? can have a significant effect on IHC results. We have tested common variations in antigen retrieval buffers, boiling devices, …

Read on for a clear IHC step-by-step guide, troubleshooting tips, and best practices to help you confidently achieve precise, high-quality results. Here’s everything you need to carry out our …