1. Follow preferred method development scheme and do “hands-on” method development – based on selectivity-changing parameters— e g pH column or mobile phase typese.g., pH, column, or mobile …

Jun 9, 2025 · In this article, you’ll discover a step-by-step guide to HPLC method development, covering everything from initial planning and separation optimisation to detection, finalisation, and real-world …

1. Theory of liquid chromatography High performance liquid chromatography (HPLC) is a technique for separating analytes dissolved in a liquid, mobile phase by using their specific interaction with a …

HPLC Method Development Step by Step Developing a robust, reproducible, and reliable HPLC or UHPLC method can be cumbersome even for an experienced liquid chromatographer.

Nov 21, 2023 · This paper provides an overview of the development and validation of High-Performance Liquid Chromatography (HPLC) methods. It addresses the construction of HPLC methods for …

Jan 29, 2024 · However, optimizing and validating an HPLC method for specific analytes requires an intricate adjustment of parameters. This review provides a comprehensive overview of the key steps …

Feb 28, 1997 · This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid …

We will explore reasons for why some methods are quite difficult to transfer to a different HPLC system. In addition, we will look at gradient method development and how to efficiently use a scouting …

Jan 29, 2025 · What’s your next step in method development? Whether you’re optimizing an existing method or starting from scratch, these strategies can help you achieve efficient, accurate, and …

Thermo Fisher Scientific offers comprehensive HPLC and UHPLC method development solutions to simplify your journey. Flexible method scouting hardware plus a suite of software tools deliver rapid, …