Tandem mass spectrometry is a powerful tool in proteomics. By ionizing peptides and then fragmenting those ions into smaller product ions, researchers can determine the biomolecules’ amino acid ...

Database searching is an essential element of large-scale proteomics. Because these methods are widely used, it is important to understand the rationale of the algorithms. Most algorithms are based on ...

Mass spectrometry (MS) has long been regarded as a cornerstone technique for biomolecular analysis, celebrated for its precision, sensitivity and ability to handle complex samples. By accurately ...

A detailed examination of top-down proteomics and its critical role in comprehensive proteoform identification using advanced mass spectrometry techniques.

TOF-SIMS is a mass spectrometry technique in which a solid sample is bombarded by ions, which then release fragments of the surface molecules. The material ejected from the sample typically is from ...

For glycopeptide characterization, mass spectrometry has arisen as the go-to methodology. As well as the instrumental arrangement, a suitable software solution is essential to enable glycopeptide ...

This is the fourth and final article in the series, describing the characterization of protein-based bio-therapeutics by bottom-up analysis at the peptide level (peptide-mapping analysis). This ...

What is the mechanism of PASEF? PASEF traps precursor ions eluted during liquid chromatography in a tunnel, where they are accumulated and ordered depending on their collisional cross-sectional area ...

After three years of development, the National Institute of Standards and Technology (NIST) has released a major upgrade of the widely used NIST/EPA/NIH Mass Spectral Library. The library is an ...